President Vladimir Putin on Wednesday sternly warned the West against encroaching further on Russia’s security interests, saying Moscow’s response will be "quick and tough" and make the culprits bitterly sorry for their action.

The warning during Putin’s annual state-of-the-nation address came amid a massive Russian military buildup near Ukraine, where cease-fire violations in the seven-year conflict between Russia-backed separatists and Ukrainian forces have escalated in recent weeks. The United States and its allies have urged the Kremlin to pull the troops back.

"I hope that no one dares to cross the red line in respect to Russia, and we will determine where it is in each specific case," Putin said. "Those who organize any provocations threatening our core security interests will regret their deeds more than they regretted anything for a long time."

Moscow has rejected Ukrainian and Western concerns about the troop buildup, saying it doesn’t threaten anyone and that Russia is free to deploy its forces on its territory. But the Kremlin also has warned Ukraine against trying to use force to retake control of the rebel-held east, saying Russia could be forced to intervene to protect civilians in the region.

"We really don’t want to burn the bridges," Putin said. "But if some mistake our good intentions for indifference or weakness and intend to burn or even blow up those bridges themselves, Russia’s response will be asymmetrical, quick and tough."

As Putin spoke, a wave of protests started rolling across Russia in support of imprisoned opposition leader Alexei Navalny, and a human rights group said nearly 1,500 people were arrested. Thousands marched in central Moscow, where police blocked off a square next to the Kremlin. Police in St. Petersburg blocked off Palace Square, outside the Hermitage museum, and protesters instead massed along Nevsky Prospekt.

The politician, who is Putin’s most persistent critic and was poisoned with a chemical nerve agent last year, started a hunger strike three weeks ago to protest what he said was inadequate medical treatment and officials’ refusal to allow his doctor to visit him. His supporters called the rallies as his health reportedly is in severe decline.

In his speech, Putin pointed to Russia’s moves to modernize its nuclear arsenal and said the military would continue to build more state-of-the-art hypersonic missiles and other new weapons. He added that the development of the nuclear-armed Poseidon underwater drone and the Burevestnik nuclear-powered cruise missile is continuing successfully.

In an apparent reference to the U.S. and its allies, the Russian leader denounced those who impose "unlawful, politically motivated economic sanctions and crude attempts to enforce its will on others." He said Russia has shown restraint and often refrained from responding to "openly boorish" actions by others.

US POISED TO ANNOUNCE SANCTIONS ON RUSSIA: REPORT

The Biden administration last week imposed new sanctions on Russia for interfering in the 2020 U.S. presidential election and for involvement in the SolarWind hack of federal agencies — activities Moscow has denied. The U.S. ordered 10 Russian diplomats expelled, targeted dozens of companies and individuals, and imposed new curbs on Russia’s ability to borrow money.

Russia retaliated by ordering 10 U.S. diplomats to leave, blacklisting eight current and former U.S. officials, and tightening requirements for U.S. Embassy operations.

"Russia has its own interests, which we will defend in line with the international law," Putin said during Wednesday’s address. "If somebody refuses to understand this obvious thing, is reluctant to conduct a dialogue and chooses a selfish and arrogant tone, Russia will always find a way to defend its position."

In an emotional outburst, Putin chastised the West for acquiring a defiant stance toward Russia.

"Some countries have developed a nasty habit of bullying Russia for any reason or without any reason at all. It has become a new sport," he said.

In an apparent reference to the U.S. allies, he compared them to Tabaqui, a cowardly golden jackal kowtowing to Shere Khan, the tiger in Rudyard Kipling’s "Jungle Book." "They howl to please their lord," he said.

Russia this week engaged in a tense tug-of-war with the Czech Republic, following Prague’s move to expel 18 Russian diplomats over a massive Czech ammunition depot explosion in 2014. Moscow has dismissed the Czech accusations of its involvement in the blast as absurd and retaliated by expelling 20 Czech diplomats.

Putin also harshly criticized the West for failing to condemn what he described as a botched coup attempt and a failed plot to assassinate Belarus’ President Alexander Lukashenko, allegedly involving a blockade of the country’s capital, power cuts and cyberattacks. Belarusian and Russian security agencies arrested the alleged coup plotters in Moscow earlier this month.

"The practice of organizing coups and planning political assassinations of top officials goes over the top and crosses all boundaries," Putin said, drawing parallels to plots against Venezuelan President Nicolas Maduro and the popular protests that led to the ouster of Ukraine’s former Russia-friendly president, Viktor Yanukovych, in 2014.

Russia responded to Yanukovych’s ouster by annexing Ukraine’s Crimean Peninsula and throwing its support to the separatists in the country’s east. Since then, fighting there has killed more than 14,000 people and devastated the industrial heartland.

CLICK HERE TO GET THE FOX NEWS APP

Putin dedicated most of his annual address to domestic issues, hailing the nation’s response to the coronavirus pandemic. He said the quick development of three coronavirus vaccines underlined Russia’s technological and industrial potential. He called for a quicker pace of immunizations, voicing hope the country could achieve collective immunity this fall.

He put forward incentives to help the economy recover from the pandemic and promised new social payments focusing on families with children.