First Mission, one of the early favorites of the Preakness Stakes, has been scratched from the second leg of the Triple Crown following concern over a potential ankle issue, officials said Friday. 

The announcement came just 36 hours ahead of the Preakness after vets at the Pimlico Race Course became concerned about an "unspecified left hind ankle issue," the Maryland Jockey Club said in a press release early Friday morning. 

First Mission ahead the Preakness

First Mission is groomed after working out on the track Tuesday morning at Pimlico Race Course in preparation for Saturday's Preakness.  (Jerry Jackson/Baltimore Sun/Tribune News Service via Getty Images)

"There was just a little concern by the 1/ST racing veterinarian team," Godolphin USA bloodstock director Michael Banahan said in a statement provided by the Maryland Jockey Club. 

HORSE EUTHANIZED AT CHURCHILL DOWNS AFTER BROKEN LEG; BECOMES 8TH THOROUGHBRED TO DIE AT TRACK IN LAST 2 WEEKS

"They thought maybe it was a minor issue with the left hind ankle. You just really couldn’t do proper diagnostics onsite, on the track," Banahan said, adding that the horse had been taken to a hospital in Lexington for further evaluation. 

First Mission during a training session ahead Preakness

First Mission goes over the track during a training session ahead of the 148th Running of the Preakness Stakes at Pimlico Race Course on May 18, 2023, in Baltimore, Maryland.  (Patrick Smith/Getty Images)

​​"It’s unfortunate, but we know he’s a very talented horse, and I’m sure we’ll have big days down the road with him."

CLICK HERE FOR MORE SPORTS COVERAGE ON FOXNEWS.COM

First Mission was the second favorite heading into the race this weekend, with Kentucky Derby winner, Mage, still the overall favorite to win with 8–5 odds. 

Friday’s news comes just days after another horse was euthanized at Churchill Downs following a leg injury. It was the eighth horse to die in the last two weeks at the famous racetrack – seven died in the lead-up to the Kentucky Derby. 

First Mission during a training session ahead Preakness

Preakness Stakes contender First Mission trains Tuesday morning at Pimlico Race Track.  (GREGORY FISHER-USA TODAY SPORTS)

CLICK HERE TO GET THE FOX NEWS APP

The number of deaths has prompted groups like PETA to call for stronger protocols to ensure the safety of the animals. 

The Associated Press contributed to this report.